15
Nov

Their imaging condition is

For fluorescence imaging, the halogen lamp was used with two filter cubes, one with excitation (BP520-540HQ) and emission (BP555-600HQ) filters for observing mKO2 fluorescence, and the other with excitation (470DF35) and emission (510WB40) filters for observing mAG fluorescence.

mKO is from http://www.biochemj.org/bj/381/0307/3810307.pdf

mAG is from http://www.jbc.org/content/278/36/34167.full.pdf

It is very safe to treat mAG as EGFP. But there is a problem with mKO. It is orange but not red. It can be excited a little by the 488 laser, which is used to excite FITC/EGFP! I don't think it is possible to overcome the problem, especially when the red is strong and the green is weak. Do you?

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