Weekly Tweets for 2011-02-28

• THERE ARE ALWAYS THINGS I COULD NEVER UNDERSTAND. Oh man, it is science, which supposes to have only one truth! #
• 雪地暴走两个小时的后果就是：全身酸痛，肩膀出血（扛雪板处），见到床就想倒下睡 #
• 高山速降两个整天的后果就是：走路时不时就觉着要转z型弯，不然速度太快，会摔…… #
• 我已经残了，这个周末的大雪暴是赶不上了——城里的雨已经来了，山上应该是一片皑皑了吧。不过，雪板新上的蜡才顶了一天，我已经顶了两天了，是该休息些日子了，呼呼 #
• 貌似twitter去facebook的同步已经费了几个星期了……怎么能把我的公共短信转到脸书墙上去呢？ #
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• oops, new macbook pro no longer uses GeForce?? #
• 用twitter/微薄，是因为我有句话要和这个世界说；用facebook/校内，是因为我有句话要和朋友们说；那我有句话要和你说该用什么呢？ #
• PMID: 21331046 @NatureNews: Fig.1b no control for cytoplasmic staining - I could get the image for any protein by moving up the focus plane #
• PMID: 21331046 @NatureNews: No protein stability estimate - I bet their delta N mutant is not stable. Not sure about SIMmut, K11A/R13A #
• PMID: 21331046 @NatureNews: Fig.5b - use IP Actin to show actin binding activity - Never seen in literatures! With EGTA, filaments all gone. #
• PMID: 21331046 @NatureNews: In buffer with EGTA, all actin filaments are disassembled. Such robust G-actin binding, why not in cytoplasmic? #
• PMID: 21331046 @NatureNews: Based on exp details, it is G-actin binds to promoters. But the claim is a mechanism involving oligomeric actin #
• PMID: 21331046 @NatureNews: Why using 10 uM NEM in the buffer for co-IP and ChIP? Since all vesicles were broken in the buffer with NP-40... #
• PMID: 21331046 @NatureNews: ... not block vesicular transport. NEM will react with all Cysteines on proteins in lysate! Are results reliable? #
• PMID: 21331046 @NatureNews: No comments on NCoR clearance, SUMO, LXR, CaMKII, etc. I am not an expert of those experiments. #
• early tester wanted, for Firefox only; https://addons.mozilla.org/en-US/firefox/addon/thepaperlinkforpubmed/ #
• lunch, lunch, lunch ... #
• 家庭暴力？打是亲，骂是爱，不打不骂是祸害 http://t.co/pUxukSY #
• I only know that @Corning makes good plastic for biomedical research "A Day Made of Glass" http://www.youtube.com/watch?v=6Cf7IL_eZ38 #

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