冰火两重天——用酶的冰与火

T4 Polynucleotide Kinase

Usage notes:
...
The efficiencies of blunt and recessed 5´-end phosphorylation can be improved by heating to 70°C for 5 minutes, then chilling on ice prior to kinase addition and by adding PEG-8,000 to 5% (w/v) (2).
...

刚刚要加酶，扫到了这条以前没有注意的
哈哈
那么我就让TPK也来爽一把吧
——毕竟
只有她爽了我才能爽啊
嗯！

碰撞，冰与火，给我力量