Nature Methods
Published online: 13 December 2009 | DOI: 10.1038/nmeth.1410Single-gene transgenic mouse strains for reprogramming adult somatic cells
Bryce W Carey 1,2, Styliani Markoulaki 1, Caroline Beard 1, Jacob Hanna 1 & Rudolf Jaenisch 1,2
We report transgenic mouse models in which three or four reprogramming factors are expressed from a single genomic locus using a drug-inducible transgene. Multiple somatic cell types can be directly reprogrammed to generate induced pluripotent stem cells (iPSCs) by culture in doxycycline. Because reprogramming factors are carried on a single polycistronic construct, the mice can be easily maintained, and the transgene can be easily transferred into other genetic backgrounds.
Whitehead Institute for Biomedical Research, Cambridge, Massachusetts, USA.
Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.Correspondence to: Rudolf Jaenisch 1,2 e-mail: jaenisch@wi.mit.edu
Insert the OSKM into collagen type I gene; cross with rtTA mice; isolate adult cells, add doxycycline for reprogramming.
What will happen, if directly feed the mice (rtTA(2):4F2A(2)) with doxycycline? Increased spontaneous tumor formation? Or, super-regeneration capability? ......
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