http://en.dogeno.us/2009/03/piggybac-transposition-reprograms-induced-pluripotent-stem-cells-in-a-safer-way/ thoughts about life and science, blogged by Liang Cai | cail.cn Wed, 08 Feb 2012 18:51:15 +0000 hourly 1 http://wordpress.org/?v=3.3.1 http://en.dogeno.us/2009/03/piggybac-transposition-reprograms-induced-pluripotent-stem-cells-in-a-safer-way/#comment-16871 Cre out the iPS factors to produce viral-free patient-derived induced pluripotent stem cells | En.dogeno.us Wed, 11 Mar 2009 02:16:37 +0000 http://en.dogeno.us/?p=3174#comment-16871 [...] I prefer the piggyBac + multicistron method to produce “safe” iPS. Although you can Cre out the viral sequence, you will left one [...] [...] I prefer the piggyBac + multicistron method to produce “safe” iPS. Although you can Cre out the viral sequence, you will left one [...]

]]> http://en.dogeno.us/2009/03/piggybac-transposition-reprograms-induced-pluripotent-stem-cells-in-a-safer-way/#comment-16852 cail Sun, 08 Mar 2009 07:13:34 +0000 http://en.dogeno.us/?p=3174#comment-16852 Hi Kevin, which paper you are referring to? The only other non-viral iPS method I know is Yamanaka's repeated DNA transfection method Hi Kevin, which paper you are referring to? The only other non-viral iPS method I know is Yamanaka's repeated DNA transfection method

]]> http://en.dogeno.us/2009/03/piggybac-transposition-reprograms-induced-pluripotent-stem-cells-in-a-safer-way/#comment-16850 kevin Sun, 08 Mar 2009 03:45:35 +0000 http://en.dogeno.us/?p=3174#comment-16850 Thank you for your introduction of this paper. non-viral vectors seem to be the future of gene therapy designed to treat a variety of human diseases. I just wonder how Nagy could publish a paper in nature given that a similar one was just published only 2 months ago in the same journal. Even there are many differences, they are describing the same strategy. What do you think? Thank you for your introduction of this paper. non-viral vectors seem to be the future of gene therapy designed to treat a variety of human diseases. I just wonder how Nagy could publish a paper in nature given that a similar one was just published only 2 months ago in the same journal. Even there are many differences, they are describing the same strategy. What do you think?

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