Comments on: my notes of the ASCB 2008 - San Francisco http://en.dogeno.us/2008/12/my-notes-of-the-ascb-2008-san-francisco/ thoughts about life and science, blogged by Liang Cai | cail.cn Wed, 08 Feb 2012 18:51:15 +0000 hourly 1 http://wordpress.org/?v=3.3.1 By: cail http://en.dogeno.us/2008/12/my-notes-of-the-ascb-2008-san-francisco/#comment-17434 cail Fri, 21 Aug 2009 21:09:30 +0000 http://en.dogeno.us/?p=2835#comment-17434 J. Brugge's paper is now online Nature. 2009 Aug 19. <b> Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment.</b> Schafer ZT, Grassian AR, Song L, Jiang Z, Gerhart-Hines Z, Irie HY, Gao S, Puigserver P, Brugge JS. [1] Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA [2] Present address: Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556, USA. Normal epithelial cells require matrix attachment for survival, and the ability of tumour cells to survive outside their natural extracellular matrix (ECM) niches is dependent on acquisition of anchorage independence. Although apoptosis is the most rapid mechanism for eliminating cells lacking appropriate ECM attachment, recent reports suggest that non-apoptotic death processes prevent survival when apoptosis is inhibited in matrix-deprived cells. Here we demonstrate that detachment of mammary epithelial cells from ECM causes an ATP deficiency owing to the loss of glucose transport. Overexpression of ERBB2 rescues the ATP deficiency by restoring glucose uptake through stabilization of EGFR and phosphatidylinositol-3-OH kinase (PI(3)K) activation, and this rescue is dependent on glucose-stimulated flux through the antioxidant-generating pentose phosphate pathway. Notably, we found that the ATP deficiency could be rescued by antioxidant treatment without rescue of glucose uptake. This rescue was found to be dependent on stimulation of fatty acid oxidation, which is inhibited by detachment-induced reactive oxygen species (ROS). The significance of these findings was supported by evidence of an increase in ROS in matrix-deprived cells in the luminal space of mammary acini, and the discovery that antioxidants facilitate the survival of these cells and enhance anchorage-independent colony formation. These results show both the importance of matrix attachment in regulating metabolic activity and an unanticipated mechanism for cell survival in altered matrix environments by antioxidant restoration of ATP generation. PMID: 19693011 J. Brugge's paper is now online

Nature. 2009 Aug 19.

Antioxidant and oncogene rescue of metabolic defects caused by loss of matrix attachment.

Schafer ZT, Grassian AR, Song L, Jiang Z, Gerhart-Hines Z, Irie HY, Gao S, Puigserver P, Brugge JS.

[1] Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115, USA [2] Present address: Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana 46556, USA.

Normal epithelial cells require matrix attachment for survival, and the ability of tumour cells to survive outside their natural extracellular matrix (ECM) niches is dependent on acquisition of anchorage independence. Although apoptosis is the most rapid mechanism for eliminating cells lacking appropriate ECM attachment, recent reports suggest that non-apoptotic death processes prevent survival when apoptosis is inhibited in matrix-deprived cells. Here we demonstrate that detachment of mammary epithelial cells from ECM causes an ATP deficiency owing to the loss of glucose transport. Overexpression of ERBB2 rescues the ATP deficiency by restoring glucose uptake through stabilization of EGFR and phosphatidylinositol-3-OH kinase (PI(3)K) activation, and this rescue is dependent on glucose-stimulated flux through the antioxidant-generating pentose phosphate pathway. Notably, we found that the ATP deficiency could be rescued by antioxidant treatment without rescue of glucose uptake. This rescue was found to be dependent on stimulation of fatty acid oxidation, which is inhibited by detachment-induced reactive oxygen species (ROS). The significance of these findings was supported by evidence of an increase in ROS in matrix-deprived cells in the luminal space of mammary acini, and the discovery that antioxidants facilitate the survival of these cells and enhance anchorage-independent colony formation. These results show both the importance of matrix attachment in regulating metabolic activity and an unanticipated mechanism for cell survival in altered matrix environments by antioxidant restoration of ATP generation.

PMID: 19693011

]]> By: a multifunctional actin assembly factor, JMY, in and out of the nucleus | En.dogeno.us http://en.dogeno.us/2008/12/my-notes-of-the-ascb-2008-san-francisco/#comment-16914 a multifunctional actin assembly factor, JMY, in and out of the nucleus | En.dogeno.us Tue, 17 Mar 2009 20:27:19 +0000 http://en.dogeno.us/?p=2835#comment-16914 [...] This work has been presented in last year’s ASCB meeting. [...] [...] This work has been presented in last year’s ASCB meeting. [...]

]]> By: jim http://en.dogeno.us/2008/12/my-notes-of-the-ascb-2008-san-francisco/#comment-16581 jim Thu, 25 Dec 2008 04:27:07 +0000 http://en.dogeno.us/?p=2835#comment-16581 Hope you are having fun skiing. Looking forward to the rest of the meeting report, very helpful. Hope you are having fun skiing. Looking forward to the rest of the meeting report, very helpful.

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